The Phospho-Stathmin 1-S25 Antibody (CABP0220) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, specifically detects the phosphorylated form of Stathmin at serine 25 in human samples and is validated for use in Western blot applications. By targeting this specific phosphorylation site, researchers can gain insight into the regulation of Stathmin activity and its impact on cellular processes.Stathmin is known for its role in regulating microtubule dynamics, which are essential for various cellular functions such as cell division, cell shape maintenance, and intracellular transport. Phosphorylation of Stathmin at serine 25 has been linked to changes in microtubule organization and dynamics, impacting cell migration, cytoskeletal reorganization, and cell signaling pathways.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-Stathmin 1-S25 Antibody
SKU:
CABP0220
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
ILSP RS
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:100 - 1:500
IHC-P
1:50 - 1:100
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
3925
RRID
AB_2771583
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using Phospho-Stathmin 1-S25 Rabbit pAb (CABP0220) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Western blot analysis of various lysates, using Phospho-Stathmin 1-S25 Rabbit pAb (CABP0220) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
