The Phospho-Smad1-S206 Antibody (CABP0295) is a high-quality antibody developed for reliable detection and analysis of target proteins. SMAD1 is a key protein involved in the TGF-β signaling pathway, playing a critical role in cell growth, differentiation, and development. This antibody, raised in rabbits, is highly specific to the phosphorylated form of SMAD1 and is validated for use in Western blot and immunohistochemistry applications.Phosphorylation of SMAD1 at serine 206 is known to regulate its activity and influence downstream signaling events in the TGF-β pathway. By specifically targeting this phosphorylation site, researchers can investigate the functional consequences of SMAD1 activation and its impact on cell behavior.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-Smad1-S206 Antibody
SKU:
CABP0295
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
PHSP T
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.
Purification Method
Affinity purification
Gene ID
4086
RRID
AB_2771538
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from C6 cells, using Phospho-Smad1-S206 Rabbit pAb (CABP0295) at 1:1000 dilution. C6 cells were treated with UV at room temperature for 15-30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
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