The Phospho-GSK3alpha-S21 Antibody (CABP0582) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.GSK3A is a key regulator of various cellular processes, including cell proliferation, differentiation, and apoptosis. Phosphorylation of GSK3A at serine 21 is known to inhibit its activity, impacting downstream signaling pathways involved in cell growth and survival. The Phospho-GSK3A (S21) Polyclonal Antibody enables detection and analysis of phosphorylated GSK3A in a variety of cell types, making it an ideal tool for studies in cell biology, cancer research, and drug development.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-GSK3alpha-S21 Antibody
SKU:
CABP0582
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
TSSF A
Tested Applications:
WBIPELISA
Recommended Dilution:
WB
1:500 - 1:2000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GSK3 alpha, GSK3A, Phospho-GSK3α-S21
Positive Sample:
293 treated with Insulin, HeLa treated with Insulin
This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease.
Purification Method
Affinity purification
Gene ID
2931
RRID
AB_2771150
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from 293 and HeLa cells, using Phospho-GSK3α-S21 Rabbit pAb (CABP0582) at 1:1000 dilution. 293 cells were treated with Insulin (100nM) for 10 minutes after serum-starvation overnight. HeLa cells were treated with Insulin (0.01U/mL) for 15 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA.
Western blot analysis of lysates from 293 cells, using Phospho-GSK3α-S21 Rabbit pAb (CABP0582) at 1:1000 dilution or GSK3α antibody (CAB0645). 293 cells were treated with Insulin (100nM) for 10 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA.
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