The Phospho-Camk2a-T286 Antibody (CABP0255) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity towards human samples and is validated for use in Western blot applications. It specifically recognizes the phosphorylated form of CaMK2A at threonine 286, allowing for the detection and analysis of this important signaling molecule in different cell types.CaMK2A, a member of the calcium/calmodulin-dependent protein kinase family, is known to play a crucial role in neuronal functions and synaptic plasticity. Phosphorylation of CaMK2A at threonine 286 is associated with its activation and involvement in various cellular pathways.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-Camk2a-T286 Antibody
SKU:
CABP0255
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
QETV D
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:2000 - 1:4000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Several transcript variants encoding distinct isoforms have been identified for this gene.
Purification Method
Affinity purification
Gene ID
816 817 818 815
RRID
AB_2770956
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse brain, using Phospho-Camk2-T286 Rabbit pAb (CABP0255) at 1:4000 dilution. Mouse brain cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
