Description
| Product Name: | Phl p 5 ELISA 2.0 kit - Single plate pack |
| SKU: | SEIB00011 |
| Antibodies: | 1D11 / Bo1 |
| Standard Range: | 3.13-200ng/mL |
| Limit of Detection: | 0.98 ng/mL |
| Background: | OD < 0.08 at 450 nm |
| Coefficient of Determination: | R² > 0.98 |
| Contents: | Plate: Pre-coated with anti-Phl p 5 monoclonal antibody 1D11 Vial 1 (white top): Phl p 5a allergen standard (2500 ng/mL) Vial 2 (brown): Biotinylated monoclonal antibody Bo1 Vial 3 (blue top): Streptavidin-peroxidase Bottle 1: Wash buffer (10× concentrate) Bottle 2: Assay buffer (10× concentrate) Bottle 3: TMB developing substrate Bottle 4: Stop solution (0.5N sulfuric acid) |
| Storage: | Store at 2–8 °C |
| Expiry: | See product insert |
| Not Provided: | Type I ultrapure or 18.2 MΩ de-ionized water Volumetric measuring equipment (serological pipette, cylinder) Clean containers for buffer/reagent preparation Calibrated single & multi-channel micropipettes and tips Vortex mixer Plate reader (450 nm) Analysis software (recommended) |
| Notes: | The allergen standard is recommended for immunoassay calibration purposes only. FAQs and troubleshooting guide available under the ‘Support’ tab on our website. |
| Pre-coated Plate: | 96-well polystyrene microtiter plate coated with monoclonal antibody 1D11 and treated with stabilizing agent. Sealed in foil pouch with desiccant. |
| Monoclonal Antibody: | 1D11 (clone 1D11 C8) |
| Immunogen: | Timothy grass pollen extract |
| Isotype: | Mouse IgG1 |
| Specificity: | Binds to a species-specific epitope on Timothy grass pollen allergen Phl p 5a & 5b. |
| Purification: | Produced in tissue culture and purified by chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE. |
| Detection Antibody: | Bo1 |
| Immunogen: | Crude Timothy grass pollen extract |
| Isotype: | Mouse IgG1 |
| Specificity: | Binds to species-specific epitope present on Timothy grass pollen allergen Phl p 5a & 5b. |
| Purification: | From ascites and purified by affinity chromatography using Protein A. Single heavy and light chain bands on SDS-PAGE. |
| Biotinylation: | Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS (pH 7.4), 0.22 μm filtered, preservative free. |
| Allergen Standard: | Recombinant Phl p 5a prepared in 1% BSA/50% glycerol/PBS (pH 7.4). |
| Concentration: | 2500 ng/mL (The rPhl p 5a was from E. coli and purified by conventional biochemical methods. Validity of the rPhl p 5 standard was confirmed by comparison with the European Pharmacopoeia reference CRS (Y0001566), recombinant major allergen rPhl p 5 containing 8.56 μg of rPhl p 5 per vial.) |
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