The Podoplanin Antibody (CAB13261) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in techniques such as immunohistochemistry, immunofluorescence, and flow cytometry.Podoplanin is commonly overexpressed in cancer cells, particularly in squamous cell carcinomas, making it a potential target for cancer therapy development. The PDPN Polyclonal Antibody binds specifically to podoplanin, enabling precise detection and analysis in different tissues and cell types.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Podoplanin Antibody
SKU:
CAB13261
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
ASTG QPED DTET TGLE GGVA MPGA EDDV VTPG TSED RYKS GLTT LVAT SVNS VTGI RIED LPTS ESTV HAQE QSPS ATAS NVAT SHST EKVD GDTQ TTVE KDGL STVT L
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:1000
IHC-P
1:20 - 1:50
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Cell Projection, Membrane, Single-Pass Type I Membrane Protein, Filopodium Membrane, Lamellipodium Membrane, Microvillus Membrane, Ruffle Membrane.
Calculated MW:
17kDa
Observed MW:
36kDa
This gene encodes a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. The specific function of this protein has not been determined but it has been proposed as a marker of lung injury. Alternatively spliced transcript variants encoding different isoforms have been identified.
Purification Method
Affinity purification
Gene ID
10630
RRID
AB_2760114
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Podoplanin Rabbit pAb (CAB13261) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human colon using Podoplanin Rabbit pAb (CAB13261) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
