The NCSTN Antibody (CAB0128) is a high-quality antibody developed for reliable detection and analysis of target proteins. It is an essential tool for researchers studying Nicastrin, a key component of the gamma-secretase complex involved in the proteolytic processing of various transmembrane proteins, including amyloid precursor protein implicated in Alzheimer's disease. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for Western blot applications.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NCSTN Antibody
SKU:
CAB0128
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ATAG1874, NCSTN
Positive Sample:
C6
Cellular Localization:
Melanosome, Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
78kDa
Observed MW:
100-120kDa
This gene encodes a type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly. The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease; however, the nature of the encoded protein's role in Alzheimer's disease is not known for certain. Mutations in this gene are associated with familial acne inversa. A pseudogene of this gene is present on chromosome 21. Alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
Purification Method
Affinity purification
Gene ID
23385
RRID
AB_2756966
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from C6 cells, using NCSTN Rabbit pAb (CAB0128) at 1:900 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunofluorescence analysis of NIH-3T3 cells using NCSTN Rabbit pAb (CAB0128) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
