p47phox/NCF1 Rabbit Polyclonal Antibody (CAB1148)

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SKU:
CAB1148
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
CGD1, NCF1A, NOXO2, p47phox, SH3PXD1A, p47phox/NCF1
Research Use:
Cell Biology
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Description

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p47phox/NCF1 Antibody (CAB1148)

The p47phox/NCF1 Antibody (CAB1148) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced from rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the NCF1 protein, researchers can detect and analyze NCF1 levels in various cell types, making it a valuable tool for studies in immunology and related fields.NCF1, also known as neutrophil cytosolic factor 1, plays a crucial role in the production of reactive oxygen species in phagocytes, which are essential for microbial killing.

This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:p47phox/NCF1 Antibody
SKU:CAB1148
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MGDT FIRH IALL GFEK RFVP SQHY VYMF LVKW QDLS EKVV YRRF TEIY EFHK TLKE MFPI EAGA INPE NRII PHLP APKW FDGQ RAAE NRQG TLTE YCST LMSL PTKI SRCP HLLD FFKV RPDD LKLP TDNQ TKKP ETYL MPKD GKST ATDI TGPI ILQT YRAI ANYE KTSG SEMA LSTG DVVE VVEK SESG WWFC QMKA KRGW IPAS FLEP LDSP DETE DPEP NYAG EPYV AIKA YTAV EGDE VSLL EGEA VEVI HKLL DGWW VIRK DDVT GYFP SMYL QKSG QDVS QAQR QIKR GAPP RRSS IRNA HSIH QRSR KRLS QDAY RRNS VRFL QQRR RQAR PGPQ SPGS PLEE ERQT QRSK PQPA VPPR PSAD LILN RCSE STKR KLAS AV
Tested Applications:WB IF/ICC IP ELISA
Recommended Dilution:
WB1:500 - 1:1000
IF/ICC1:50 - 1:100
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CGD1, NCF1A, NOXO2, p47phox, SH3PXD1A, p47phox/NCF1
Positive Sample:Raji, Daudi, Jurkat(Negative control), Raji
Cellular Localization:Cytoplasm, Cytoplasmic Side, Membrane, Peripheral Membrane Protein, Cytosol.
Calculated MW:45kDa
Observed MW:47kDa

The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease.

Purification MethodAffinity purification
Gene ID653361
RRIDAB_2758579
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates, using p47phox/NCF1 Rabbit pAb (CAB1148) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Product ImageWestern blot analysis of lysates from Raji cells using p47phox/NCF1 Rabbit pAb (CAB1148) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time:10s.
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