The MTCO1 Antibody (CAB17889) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, displays high reactivity with human samples and is suitable for use in Western blot applications.The MTCO1 protein plays a crucial role in mitochondrial function and energy production, making it a key target for studies in areas such as metabolism, aging, and neurodegenerative diseases. By detecting and analyzing the MTCO1 protein in various cell types, this antibody enables researchers to explore the role of COX subunits in cellular respiration and mitochondrial dysfunction.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MTCO1 Antibody
SKU:
CAB17889
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
COI, MTCO1, COX1
Positive Sample:
THP-1, HepG2, HeLa, Mouse liver, Rat kidney
Cellular Localization:
Mitochondrial Inner Membrane, Mitochondrial Respiratory Chain Complex Iii, Mitochondrial Respiratory Chain Complex Iv.
Calculated MW:
57kDa
Observed MW:
40kDa/37kDa
Contributes to cytochrome-c oxidase activity. Predicted to be involved in electron transport coupled proton transport and mitochondrial electron transport, cytochrome c to oxygen. Part of mitochondrial respiratory chain complex III and mitochondrial respiratory chain complex IV.
Purification Method
Affinity purification
Gene ID
4512
RRID
AB_2861744
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide,pH7.3.
Western blot analysis of various lysates using MTCO1 Rabbit pAb (CAB17889) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Mouse heart using MTCO1 Rabbit pAb (CAB17889) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat brain using MTCO1 Rabbit pAb (CAB17889) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat kidney using MTCO1 Rabbit pAb (CAB17889) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
