The mouse CD24 Polyclonal Antibody (CAB24878) is a high-quality antibody developed for reliable detection and analysis of target proteins. CD24 is a GPI-anchored glycoprotein and P-selectin ligand expressed on hematopoietic, neural, and epithelial cells that plays a role in cell adhesion and lymphocyte development staging. It binds Siglec-10 (human) or Siglec-G (mouse) and is widely used as a cancer stem cell marker for detecting and isolating various types of cancer cells.
This antibody is validated for use in WB, FC, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
mouse CD24 Polyclonal Antibody
SKU:
CAB24878
Size:
20μL, 100μL
Reactivity:
Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBFCELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
Mouse liver
Cellular Localization:
Cell Membrane.
Calculated MW:
8kDa
Observed MW:
Refertofigures
CD24 (also known as heat-resistant antigen HSA) is a P-selectin ligand associated with adhesion. It is a GPI anchored glycoprotein expressed in many types of cells, including hematopoietic cells, neural cells, and epithelial cells. CD24 is widely used to determine the stage of lymphocyte development. It also binds to Siglec-10 in human cells or Siglec-G in mouse cells. CD24 is often used as a cancer stem cell marker for detecting and isolating various types of cancer cells.
Purification Method
Affinity purification
Gene ID
12484
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse liver using mouse CD24 Rabbit pAb (CAB24878) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
