The Mouse anti V5-Tag Monclonal Antibody (CABE017) is a high-quality antibody developed for reliable detection and analysis of target proteins. The V5-tag is a well-characterized epitope derived from the paramyxovirus SV5, genetically fused to recombinant proteins to provide a reliable handle for immunological detection and purification. It is particularly advantageous in mammalian expression systems where its non-endogenous origin minimizes background from host cell proteins.
This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Species independent samples.
Product Name:
Mouse anti V5-Tag Monclonal Antibody
SKU:
CABE017
Size:
50μL, 100μL, 200μL
Reactivity:
Species independent
Clone Number:
AMC0506
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCIPELISA
Recommended Dilution:
WB
1:2000 - 1:10000
IF/ICC
1:50 - 1:200
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
V5, V5 tag, V5-tag
Positive Sample:
Recombinant Mouse V5 protein
Observed MW:
RefertoFigures
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
Purification Method
Affinity purification
RRID
AB_2770413
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of recombinant Mouse V5 protein using Mouse anti V5-Tag mAb (CABE017) at dilution of 1:2000. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Lysates/proteins: 10ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Confocal imaging of 293F transfected with V5 and 293F cells using Mouse anti V5-Tag mAb (CABE017, dilution 1:200) followed by a further incubation with ABflo® 488-conjugated Goat anti-Mouse IgG (H+L) (CABS076, 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
