The Mouse anti Myc-Tag Monclonal Antibody (CABE010) is a high-quality antibody developed for reliable detection and analysis of target proteins. The Myc-tag (EQKLISEEDL) is a short viral-derived epitope genetically incorporated into recombinant proteins to confer a reliable immunological handle for detection and purification. It is widely used in western blotting, immunofluorescence, and immunoprecipitation experiments due to the well-characterized specificity of anti-Myc antibodies.
This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Species independent samples.
Product Name:
Mouse anti Myc-Tag Monclonal Antibody
SKU:
CABE010
Size:
50μL, 100μL
Reactivity:
Species independent
Clone Number:
AMC0504
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCIPELISA
Recommended Dilution:
WB
1:2000 - 1:10000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
IF/ICC
1:1000-1:5000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Myc, Myc tag, Myc-tag
Positive Sample:
293F transfected with NLK-Myc (C-terminal), 293F transfected with PTEN-Myc (C-terminal)
Observed MW:
62kDa,60kDa
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
Purification Method
Affinity purification
RRID
AB_2770408
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with NLK-Myc (C-terminal) or PTEN-Myc (C-terminal) using Mouse anti Myc-Tag mAb (CABE010) at 1:10000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020) .Exposure time: 30s.
Confocal imaging of 293F cells transfected with SQSTM1-Myc(N) cells using Mouse anti Myc-Tag mAb (CABE010, dilution 1:2000) followed by a further incubation with Cy3-conjugated Goat anti-Mouse IgG (H+L) (CABS008, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
