Description
| Product Name: | Mouse Anti-CD3 Low Endotoxin In Vivo Antibody (Phenocycler-Fusion Validated) |
| SKU: | IVMB0293 |
| Size: | 50 µg |
| Antibody Type: | Monoclonal Antibody |
| Clone: | 17A2 |
| Target: | CD3 |
| Isotype: | Rat IgG2b κ |
| Host Species: | Rat |
| Reactivity: | Mouse |
| Applications: | IHC FF, PhenoCycler® |
| Synonyms: | T-cell Receptor Complex, CD3ε |
| Product Concentration: | 0.5 mg/ml |
| Immunogen: | γ/δ TCR-positive T-T hybridoma D1 |
| Antigen Distribution: | CD3 is expressed on human peripheral blood lymphocytes, splenic lymphocytes, and the majority of T-CLL and T-ALL cells. |
| Formulation: | This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4. |
| Specificity: | Clone 17A2 recognizes an epitope on mouse CD3. |
CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.
| Ligand/Receptor: | Peptide antigen/MHC-complex |
| Product Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. |
