[KO Validated] Lamin A/C Rabbit Polyclonal Antibody (CAB0249)

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SKU:
CAB0249
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
FPL, IDC, LFP, CDDC, EMD2, FPLD, HGPS, LDP1, LMN1, LMNC, MADA, PRO1, CDCD1, CMD1A, FPLD2, LMNL1, CMT2B1, LGMD1B, Lamin A/C
Research Use:
Cell Biology
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Description

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[KO Validated] Lamin A/C Antibody (CAB0249)

The [KO Validated] Lamin A/C Antibody (CAB0249) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to Lamin A/C, this antibody enables precise detection and analysis of the protein in various cell types, making it an essential tool for investigations in cell biology and developmental research.

This antibody is validated for use in WB, IHC-P, IF/ICC, ChIP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] Lamin A/C Antibody
SKU:CAB0249
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:SSHS SQTQ GGGS VTKK RKLE STES RSSF SQHA RTSG RVAV EEVD EEGK FVRL RNKS NEDQ SMGN WQIK RQNG DDPL LTYR FPPK FTLK AGQV VTIW AAGA GATH SPPT DLVW KAQN TWGC GNSL RTAL INST GEEV AMRK LVRS VTVV EDDE DEDG DDLL HHHH GSHC SS
Tested Applications:WB IHC-P IF/ICC ChIP ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
ChIP1:20 - 1:100
Synonyms:FPL, IDC, LFP, CDDC, EMD2, FPLD, HGPS, LDP1, LMN1, LMNC, MADA, PRO1, CDCD1, CMD1A, FPLD2, LMNL1, CMT2B1, LGMD1B, Lamin A/C
Positive Sample:HeLa, NIH/3T3, PC-12
Cellular Localization:Nucleus, Nucleus Envelope, Nucleus Lamina, Nucleus Speckle, Nucleoplasm.
Calculated MW:74kDa
Observed MW:68kDa/72kDa

The protein encoded by this gene is part of the nuclear lamina, a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.

Purification MethodAffinity purification
Gene ID4000
RRIDAB_2757062
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates, using Lamin A/C Rabbit pAb (CAB0249) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse brain using Lamin A/C Rabbit pAb (CAB0249) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat liver using Lamin A/C Rabbit pAb (CAB0249) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunofluorescence analysis of NIH/3T3 cells using Lamin A/C Rabbit pAb (CAB0249) at dilution of 100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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