[KO Validated] YB-1/YBX1 Rabbit Polyclonal Antibody (CAB7704)

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SKU:
CAB7704
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse, Rat
Synonyms:
YB1, BP-8, CSDB, DBPB, YB-1, CBF-A, CSDA2, EFI-A, NSEP1, NSEP-1, MDR-NF1, YB-1/YBX1
Research Use:
Epigenetics and Nuclear Signaling
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Description

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[KO Validated] YB-1/YBX1 Antibody (CAB7704)

The [KO Validated] YB-1/YBX1 Antibody (CAB7704) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is specifically designed for use in Western blot applications and has been validated for its high reactivity with human samples.YBX1, also known as Y-box binding protein 1, plays a critical role in regulating gene expression, DNA repair, and cell proliferation. It is implicated in a variety of biological processes, making it a key target for research in fields such as cancer biology, developmental biology, and molecular biology.

This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] YB-1/YBX1 Antibody
SKU:CAB7704
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:LRSV GDGE TVEF DVVE GEKG AEAA NVTG PGGV PVQG SKYA ADRN HYRR YPRR RGPP RNYQ QNYQ NSES GEKN EGSE SAPE GQAQ QRRP YRRR RFPP YYMR R
Tested Applications:WB IHC-P ELISA IF-P
Recommended Dilution:
WB1:500 - 1:2000
IF-P1:50 - 1:200
IHC-P1:100 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:YB1, BP-8, CSDB, DBPB, YB-1, CBF-A, CSDA2, EFI-A, NSEP1, NSEP-1, MDR-NF1, YB-1/YBX1
Positive Sample:HepG2
Cellular Localization:Cytoplasm, Cytoplasmic Granule, Nucleus, Secreted.
Calculated MW:36kDa
Observed MW:49kDa

This gene encodes a highly conserved cold shock domain protein that has broad nucleic acid binding properties. The encoded protein functions as both a DNA and RNA binding protein and has been implicated in numerous cellular processes including regulation of transcription and translation, pre-mRNA splicing, DNA reparation and mRNA packaging. This protein is also a component of messenger ribonucleoprotein (mRNP) complexes and may have a role in microRNA processing. This protein can be secreted through non-classical pathways and functions as an extracellular mitogen. Aberrant expression of the gene is associated with cancer proliferation in numerous tissues. This gene may be a prognostic marker for poor outcome and drug resistance in certain cancers. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on multiple chromosomes.

Purification MethodAffinity purification
Gene ID4904
RRIDAB_2863557
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from HepG2 cells, using YB-1/YBX1 Rabbit pAb (CAB7704) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Rat testis using YB-1/YBX1 Rabbit pAb (CAB7704) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human colon carcinoma using YB-1/YBX1 Rabbit pAb (CAB7704) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of paraffin-embedded mouse colon using YB-1/YBX1 Rabbit pAb (CAB7704) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of paraffin-embedded mouse brain using YB-1/YBX1 Rabbit pAb (CAB7704) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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