[KO Validated] SMARCB1/SNF5 Rabbit Monoclonal Antibody (CAB3247)

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SKU:
CAB3247
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle
Antibody Type:
Monoclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
RDT, CSS3, INI1, SNF5, Snr1, BAF47, INI-1, MRD15, RTPS1, Sfh1p, hSNFS, SNF5L1, SWNTS1, PPP1R144, F5
Research Use:
Cell Cycle
€0.00
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Description

system_update_altDatasheetsystem_update_altMSDS

[KO Validated] SMARCB1/SNF5 Monoclonal Antibody (CAB3247)

The [KO Validated] SMARCB1/SNF5 Monoclonal Antibody (CAB3247) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit monoclonal antibody has been rigorously validated for knockout validation and is highly specific to SNF5 in human samples.The antibody's superior performance in Western blot applications ensures accurate and reliable detection of SNF5, enabling researchers to explore its functions in various cellular processes. Its high sensitivity and specificity make it an excellent choice for studies in cancer biology, epigenetics, and developmental biology.

This antibody is validated for use in WB, IHC-P, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] SMARCB1/SNF5 Monoclonal Antibody
SKU:CAB3247
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Clone Number:ARC53139
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:LWRR LATV EERK KIVA SSHG KKTK PNTK DHGY TTLA TSVT LLKA SEVE EILD GNDE KYKA VSIS TEPP TYLR EQKA KRNS QWVP TLPN SSHH LDAV PCST T
Tested Applications:WB IHC-P IP ELISA
Recommended Dilution:
WB1:1000 - 1:5000
IHC-P1:50 - 1:200
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:RDT, CSS3, INI1, SNF5, Snr1, BAF47, INI-1, MRD15, RTPS1, Sfh1p, hSNFS, SNF5L1, SWNTS1, PPP1R144, F5
Positive Sample:293F, K-562, NIH/3T3, Neuro-2a, Rat testis, 293F
Cellular Localization:Nucleus.
Calculated MW:44kDa
Observed MW:44kDa

The protein encoded by this gene is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor, and mutations in it have been associated with malignant rhabdoid tumors. Alternatively spliced transcript variants have been found for this gene.

Purification MethodAffinity purification
Gene ID6598
RRIDAB_2863030
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates, using SMARCB1/SNF5 Rabbit mAb (CAB3247) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:2000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageWestern blot analysis of lysates from wild type(WT) and SMARCB1/SNF5 Rabbit mAb knockout (KO) HeLa(KO) cells, using SMARCB1/SNF5 Rabbit mAb (CAB3247) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse heart using [KO Validated] SMARCB1/SNF5 Rabbit mAb (CAB3247) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageImmunoprecipitation of from 300 µg extracts of 293F cells was performed using 3 µg of [KO Validated] SMARCB1/SNF5 Rabbit mAb (CAB3247). Rabbit IgG isotype control (AC042) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using [KO Validated] SMARCB1/SNF5 Rabbit mAb (CAB3247) at a dilution of 1:12000.
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