[KO Validated] PDCD4 Rabbit Polyclonal Antibody (CAB2570)

(No reviews yet) Write a Review
お見積り依頼
SKU:
CAB2570
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse
Synonyms:
H731, D4
Research Use:
Cell Death
€0.00
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS

[KO Validated] PDCD4 Antibody (CAB2570)

The [KO Validated] PDCD4 Antibody (CAB2570) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for Western blot applications. By targeting the PDCD4 protein, this antibody allows for accurate detection and analysis in various cell types, making it ideal for studies in molecular biology and cancer research.PDCD4, also known as programmed cell death protein 4, is a tumor suppressor that plays a crucial role in controlling cell cycle progression and apoptosis.

This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:[KO Validated] PDCD4 Antibody
SKU:CAB2570
Size:20μL, 100μL
Reactivity:Human, Mouse
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MDVE NEQI LNVN PADP DNLS DSLF SGDE ENAG TEEI KNEI NGNW ISAS SINE ARIN AKAK RRLR KNSS RDSG RGDS VSDS GSDA LRSG LTVP TSPK GRLL DRRS RSGK GRGL PKKG GAGG KGVW GTPG QVYD VEEV DVKD PNYD DDQE NCVY ETVV LPLD ERAF EKTL TPII QEYF EHGD TNEV AEML RDLN LGEM KSGV PVLA VSLA LEGK ASHR EMTS KLLS DLCG TVMS TTDV EKSF DKLL KDLP ELAL DTPR APQL
Tested Applications:WB IHC-P ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:H731, D4
Positive Sample:293T, HeLa, MCF7, Mouse thymus
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:52kDa
Observed MW:60kDa

This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants.

Purification MethodAffinity purification
Gene ID27250
RRIDAB_2863014
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using [KO Validated] PDCD4 Rabbit pAb (CAB2570) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Product ImageWestern blot analysis of lysates from wild type (WT) and PDCD4 knockout (KO) 293T cells, using [KO Validated] PDCD4 Rabbit pAb (CAB2570) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human thyroid cancer using [KO Validated] PDCD4 Rabbit pAb (CAB2570) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human placenta using [KO Validated] PDCD4 Rabbit pAb (CAB2570) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
View AllClose

View AllClose

Related Products