The [KO Validated] CSPG4 Antibody (CAB3592) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, generated in rabbits, exhibits high reactivity towards human samples and has been validated for use in Western blot applications. By binding specifically to the CSPG4 protein, this antibody enables precise detection and analysis in different cell types, making it ideal for research in neuroscience, cancer biology, and developmental biology.CSPG4, also known as NG2, is a key player in cell-matrix interactions, cell signaling pathways, and cell proliferation. Its involvement in neural development, tumor progression, and wound healing make it a versatile target for investigating a wide range of physiological and pathological processes.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] CSPG4 Antibody
SKU:
CAB3592
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Apical Cell Membrane, Cell Projection, Extracellular Side, Single-Pass Type I Membrane Protein, Lamellipodium Membrane.
Calculated MW:
251kDa
Observed MW:
250kDa-450kDa
A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
Purification Method
Affinity purification
Gene ID
1464
RRID
AB_2863096
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from A375 cells, using [KO Validated] NG2/CSPG4 Rabbit pAb (CAB3592) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of various lysates using [KO Validated] NG2/CSPG4 Rabbit pAb (CAB3592) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Western blot analysis of lysates from wild type (WT) and NG2/CSPG4 knockout (KO) HeLa cells, using [KO Validated] NG2/CSPG4 Rabbit pAb (CAB3592) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of lysates from wild type(WT) and NG2/CSPG4 Rabbit pAb knockout (KO) HeLa cells, using [KO Validated] NG2/CSPG4 Rabbit pAb (CAB3592) at 1:600 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
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