The [KO Validated] CHRAC1 Antibody (CAB14896) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, has been rigorously tested and validated for use in a variety of applications, including Western blot and immunofluorescence.CHD4, also known as Chromodomain Helicase DNA-binding Protein 4, is essential for maintaining chromatin structure and regulating gene expression. Dysregulation of CHD4 has been implicated in various diseases, including cancer and developmental disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] CHRAC1 Antibody
SKU:
CAB14896
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
CHRAC1 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.
Purification Method
Affinity purification
Gene ID
54108
RRID
AB_2861709
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and CHRAC1 knockout (KO) HeLa cells, using [KO Validated] CHRAC1 Rabbit pAb (CAB14896) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 15s.
Western blot analysis of lysates from A549 cells using CHRAC1 Rabbit pAb (CAB14896) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 10s.
![[KO Validated] CHRAC1 Rabbit Polyclonal Antibody (CAB14896)](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/608x608/products/138876/138697/anti-chrac1-antibody-cab14896ko-validated__51170__41233.1750429038.jpg?c=1 "[KO Validated] CHRAC1 Rabbit Polyclonal Antibody (CAB14896)")
![[KO Validated] CHRAC1 Rabbit Polyclonal Antibody (CAB14896)](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/1280x1280/products/138876/138697/anti-chrac1-antibody-cab14896ko-validated__51170__41233.1750429038.jpg?c=1&imbypass=on "[KO Validated] CHRAC1 Rabbit Polyclonal Antibody (CAB14896)")
