The [KO Validated] CD44 Antibody (CAB12410) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in various research applications, including Western blot and immunohistochemistry.CD44 is known to play a key role in cell-cell interactions, cell adhesion, and migration, making it an important target for studies in cancer research, stem cell biology, and immunology. With its ability to bind to the CD44 protein, this antibody enables accurate detection and analysis of CD44 expression in different cell types and tissues.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
[KO Validated] CD44 Antibody
SKU:
CAB12410
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Cell Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
82kDa
Observed MW:
80kDa
The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
Purification Method
Affinity purification
Gene ID
960
RRID
AB_2861662
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using [KO Validated] CD44 Rabbit pAb (CAB12410) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of lysates from wild type (WT) and CD44 knockout (KO) HeLa cells, using [KO Validated] CD44 Rabbit pAb (CAB12410) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using [KO Validated] CD44 Rabbit pAb (CAB12410) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of HeLa cells using [KO Validated] CD44 Rabbit pAb (CAB12410) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of RAW264.7 cells using [KO Validated] CD44 Rabbit pAb (CAB12410) at dilution of 1:100. Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of HeLa cells using [KO Validated] CD44 Rabbit pAb (CAB12410) at dilution of 1:100. Blue: DAPI for nuclear staining.
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