[KO Validated] Cyclin D3 Rabbit Polyclonal Antibody (CAB0746)

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SKU:
CAB0746
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle
Reactivity:
Human, Mouse
Synonyms:
CCND3, cyclin D3, Cyclin D3, D3
Research Use:
Cell Cycle
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Description

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[KO Validated] Cyclin D3 Antibody (CAB0746)

The [KO Validated] Cyclin D3 Antibody (CAB0746) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, specifically targets CCND3 in human samples and has been validated for use in Western blot applications. By binding to the CCND3 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential component for studies in cell biology and cancer research.CCND3, also known as cyclin D3, plays a crucial role in promoting cell cycle progression and regulating cell growth. Dysregulation of CCND3 has been implicated in tumorigenesis and cancer progression, making it a promising target for therapeutic interventions.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:[KO Validated] Cyclin D3 Antibody
SKU:CAB0746
Size:20μL, 100μL
Reactivity:Human, Mouse
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:SMIA TGSI GAAV QGLG ACSM SGDE LTEL LAGI TGTE VDCL RACQ EQIE AALR ESLR EASQ TSSS PAPK APRG SSSQ GPSQ TSTP TDVT AIHL
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:2000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CCND3, cyclin D3, Cyclin D3, D3
Positive Sample:HeLa, K-562, Mouse spleen
Cellular Localization:Cytoplasm, Membrane, Nucleus.
Calculated MW:33kDa
Observed MW:36kDa

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene.

Purification MethodAffinity purification
Gene ID896
RRIDAB_2757375
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using [KO Validated] Cyclin D3 Rabbit pAb (CAB0746) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Product ImageWestern blot analysis of lysates from wild type (WT) and Cyclin D3 knockout (KO) HeLa cells, using [KO Validated] Cyclin D3 Rabbit pAb (CAB0746) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
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