The KDM5A Antibody (CAB7238) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this antibody exhibits high specificity and sensitivity when used in Western blot applications with human samples. By binding to the KDM5A protein, researchers can accurately detect and analyze its expression levels in various cell types, making it an invaluable asset in the fields of epigenetics and cancer research.KDM5A, also known as lysine-specific demethylase 5A, plays a critical role in histone modification and gene expression regulation.
This antibody is validated for use in WB, IP, ChIP, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
KDM5A Antibody
SKU:
CAB7238
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIPChIPELISA
Recommended Dilution:
WB
1:500 - 1:2000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
ChIP
5μg antibody for 10μg-15μg of Chromatin
Synonyms:
RBP2, RBBP2, RBBP-2, KDM5A
Positive Sample:
Jurkat, HeLa, MCF7
Cellular Localization:
Nucleus, Nucleolus.
Calculated MW:
192kDa
Observed MW:
192kDa
This gene encodes a member of the Jumonji, AT-rich interactive domain 1 (JARID1) histone demethylase protein family. The encoded protein plays a role in gene regulation through the histone code by specifically demethylating lysine 4 of histone H3. The encoded protein interacts with many other proteins, including retinoblastoma protein, and is implicated in the transcriptional regulation of Hox genes and cytokines. This gene may play a role in tumor progression.
Purification Method
Affinity purification
Gene ID
5927
RRID
AB_2767786
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using KDM5A Rabbit pAb (CAB7238) at 1:500 dilution._Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 60s.
Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg KDM5A antibody (CAB7238). Western blot was performed from the immunoprecipitate using KDM5A antibody (CAB7238) at a dilution of 1:500.
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