The IVD Polyclonal Antibody (CAB23595) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this highly reactive antibody is specifically designed for use with human samples and is validated for Western blot applications. By binding to the CD300A protein, researchers can accurately detect and analyze this important molecule in various cell types, making it ideal for studies in immunology and cancer research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
IVD Polyclonal Antibody
SKU:
CAB23595
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
IVDH, ACAD2, IVD
Positive Sample:
Mouse heart
Cellular Localization:
Mitochondrion Matrix.
Calculated MW:
47kDa
Observed MW:
42kDa
Isovaleryl-CoA dehydrogenase (IVD) is a mitochondrial matrix enzyme that catalyzes the third step in leucine catabolism. The genetic deficiency of IVD results in an accumulation of isovaleric acid, which is toxic to the central nervous system and leads to isovaleric acidemia. Alternatively spliced transcript variants have been found for this gene.
Purification Method
Affinity purification
Gene ID
3712
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse heart, using IVD Rabbit pAb (CAB23595) at 1:6000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
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