The IL18BP Antibody (CAB6445) is a high-quality antibody developed for reliable detection and analysis of target proteins. IL18BP, also known as Interleukin 18 Binding Protein, functions by binding to interleukin-18 (IL-18) and inhibiting its pro-inflammatory activities.This polyclonal antibody, generated in rabbits, is highly specific and reactive with human samples, allowing for accurate detection and analysis of IL18BP in Western blot applications. Its versatility in binding to the IL18BP protein enables researchers to study its function in various cell types, making it a valuable asset for studies in immunology and inflammation research.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
IL18BP Antibody
SKU:
CAB6445
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
FVH, IL18BPa, IL18BP
Positive Sample:
Mouse thymus
Cellular Localization:
Secreted.
Calculated MW:
21kDa
Observed MW:
44kDa
The protein encoded by this gene functions as an inhibitor of the proinflammatory cytokine, IL18. It binds IL18, prevents the binding of IL18 to its receptor, and thus inhibits IL18-induced IFN-gamma production, resulting in reduced T-helper type 1 immune responses. This protein is constitutively expressed and secreted in mononuclear cells. Elevated level of this protein is detected in the intestinal tissues of patients with Crohn's disease. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Purification Method
Affinity purification
Gene ID
10068
RRID
AB_2767047
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse thymus, using IL18BP Rabbit pAb (CAB6445) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunofluorescence analysis of A549 cells using IL18BP Rabbit pAb (CAB6445). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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