The IGFBP1 Antibody (CAB2981) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the IGFBP1 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an essential tool for investigations in the fields of endocrinology and metabolism.IGFBP1 is known to play a crucial role in mediating the actions of insulin-like growth factors, which are implicated in various physiological processes such as growth, development, and metabolism.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
IGFBP1 Antibody
SKU:
CAB2981
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AFBP, IBP1, PP12, IGF-BP25, hIGFBP-1, IGFBP1
Positive Sample:
HepG2
Cellular Localization:
Secreted.
Calculated MW:
28kDa
Observed MW:
30kDa
This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP N-terminal domain and a thyroglobulin type-I domain. The encoded protein, mainly expressed in the liver, circulates in the plasma and binds both insulin-like growth factors (IGFs) I and II, prolonging their half-lives and altering their interaction with cell surface receptors. This protein is important in cell migration and metabolism. Low levels of this protein may be associated with impaired glucose tolerance, vascular disease and hypertension in human patients.
Purification Method
Affinity purification
Gene ID
3484
RRID
AB_2764792
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HepG2 cells, using IGFBP1 Rabbit pAb (CAB2981) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
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