The Icam1 Polyclonal Antibody (CAB23661) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the ICAM1 protein, this antibody enables the detection and analysis of ICAM1 expression in a wide range of cell types, making it an essential tool for studies in immunology, inflammatory diseases, and cancer research.ICAM1 plays a crucial role in mediating the adhesion between immune cells and endothelial cells, facilitating immune cell trafficking and inflammation.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
Icam1 Polyclonal Antibody
SKU:
CAB23661
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISAIF-P
Recommended Dilution:
WB
1:500 - 1:1000
IF-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CD54, ICAM, Icam1
Positive Sample:
Mouse spleen, Mouse lung, Rat spleen, Rat lung
Calculated MW:
60kDa
Observed MW:
102kDa
Enables integrin binding activity. Involved in several processes, including cellular response to cytokine stimulus; cellular response to lipid; and gonad development. Located in cell surface; extracellular space; and plasma membrane. Used to study diabetic angiopathy; mesenteric vascular occlusion; nephritis; pre-eclampsia; and uveitis. Biomarker of several diseases, including cerebrovascular disease (multiple); colitis (multiple); kidney failure (multiple); lung disease (multiple); and pancreatitis (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); biliary atresia; diabetic retinopathy; inflammatory bowel disease (multiple); and liver cirrhosis. Orthologous to human ICAM1 (intercellular adhesion molecule 1).
Purification Method
Affinity purification
Gene ID
25464
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using Icam1 Rabbit pAb (CAB23661) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
Immunofluorescence analysis of paraffin-embedded Mouse lung tissue using Icam1 Rabbit pAb (CAB23661) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
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