The ICAM-1/CD54 Polyclonal Antibody (CAB13620) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific and reactive with human samples, making it suitable for a variety of research applications.ICAM1 is a critical molecule in the immune system, functioning as a ligand for the integrin LFA-1 and playing a key role in adhesion and activation of leukocytes. It is involved in various physiological processes such as T cell activation, leukocyte recruitment, and immune response to pathogens. The ICAM1 Polyclonal Antibody binds specifically to ICAM1 protein, enabling accurate detection and analysis in different cell types.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
ICAM-1/CD54 Polyclonal Antibody
SKU:
CAB13620
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BB2, CD54, P3.58, ICAM-1/CD54
Positive Sample:
Raji
Cellular Localization:
Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
58kDa
Observed MW:
92kDa
This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor.
Purification Method
Affinity purification
Gene ID
3383
RRID
AB_2760481
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Raji cells, using ICAM-1/CD54 Rabbit pAb (CAB13620) at 1:800 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
