Human UCHL1 (Ubiquitin Carboxyl Terminal Hydrolase L1) ELISA Kit
The Human UCHL1 (Ubiquitin Carboxyl-Terminal Hydrolase L1) ELISA Kit is meticulously crafted for the precise measurement of UCHL1 levels in human samples like serum, plasma, and cell culture supernatants. This cutting-edge kit boasts unparalleled sensitivity and specificity, guaranteeing dependable and consistent results, thus rendering it optimal for a diverse array of research undertakings.UCHL1 is an integral enzyme intricately involved in the ubiquitin-proteasome pathway, crucial for protein degradation and maintenance of cellular homeostasis. Aberrations in UCHL1 expression have been linked to various diseases, including Parkinson's disease, Alzheimer's disease, and certain cancers, underscoring its significance as a key target for therapeutic interventions and disease biomarker development.
Product Name:
Human UCHL1 (Ubiquitin Carboxyl Terminal Hydrolase L1) ELISA Kit
SKU:
HUES03248
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
