The Human TP53 (Tumor Protein p53) ELISA Kit is designed for the quantitative detection of Human TP53 (Tumor Protein p53) levels in various biological samples. Tumor Protein p53 (TP53) is a sequence-specific transcription factor and master tumor suppressor that safeguards genomic integrity by activating DNA repair mechanisms, inducing cell cycle arrest, and triggering apoptosis in response to genotoxic stress. Somatic TP53 mutations are found in approximately 50% of all human cancers, representing the most frequently altered gene in human malignancy. Accurate measurement of TP53 protein levels is essential for understanding tumor biology, cancer progression, resistance to therapy, and the development of TP53-targeted anticancer strategies. Assay Genie's Human TP53 (Tumor Protein p53) ELISA Kit offers exceptional sensitivity and specificity with a sensitivity of 46.88 pg/mL, ensuring reliable and reproducible results across a broad range of sample types including serum, plasma and other biological fluids. Manufactured under stringent quality control standards, this ELISA kit provides robust performance and ease of use, making it an outstanding choice for both research and clinical applications. Trust Assay Genie's Human TP53 (Tumor Protein p53) ELISA Kit for accurate and dependable quantification of this crucial biomarker in your studies.
Product Name:
Human TP53 (Tumor Protein p53) ELISA Kit
SKU:
AEES00029
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
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