Human PLAP/ALPP (Placental Alkaline Phosphatase) ELISA Kit
The Human PLAP (ALPP) ELISA Kit is a specialized assay designed for the precise measurement of placental alkaline phosphatase levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications. Placental alkaline phosphatase is a key enzyme that plays a critical role in cellular activities and is implicated in various diseases and disorders. Studying placental alkaline phosphatase levels can provide valuable insights into conditions such as pregnancy complications, bone disorders, and certain cancers. With its reliable performance and robust design, the Human PLAP (ALPP) ELISA Kit is a valuable tool for researchers seeking to understand the implications of placental alkaline phosphatase in health and disease.
Product Name:
Human PLAP/ALPP (Placental Alkaline Phosphatase) ELISA Kit
SKU:
HUES02914
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
system_update_altDatasheetHuman Alkaline Phosphatase, Placental (ALPP) ELISA KitThe Human Alkaline Phosphatase Placental (ALPP) ELISA Kit is specifically designed for the accu
