Human NAGase (N-Acetyl Beta-D-Glucosaminidase) ELISA Kit
The Human NAGASE (N-acetyl-beta-D-glucosaminidase) ELISA Kit is a reliable tool for the precise measurement of NAGASE levels in human samples including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers consistent and accurate results, making it suitable for a diverse range of research purposes.NAGASE is an important enzyme involved in the breakdown of complex sugars, playing a key role in various biological processes. Its dysregulation has been linked to several diseases such as lysosomal storage disorders and renal diseases. By measuring NAGASE levels, researchers can gain valuable insights into these conditions and explore potential therapeutic interventions.This ELISA kit provides a convenient and efficient solution for studying NAGASE and its implications in human health and disease. Trust in its performance to advance your research and discover new possibilities in the field of biochemistry.
Product Name:
Human NAGase (N-Acetyl Beta-D-Glucosaminidase) ELISA Kit
SKU:
HUES02024
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
system_update_altDatasheetHuman N-Acetyl Beta-D-Glucosaminidase (NAGase) ELISA KitThe Human N-acetyl-beta-D-glucosaminidase (NAGase) ELISA Kit is a powerful tool for researche