Human CRH (Corticotropin Releasing Hormone) ELISA Kit
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- SKU:
- HUFI00818
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- P06850
- Sensitivity:
- 1.4pg/ml
- Range:
- 2.188-140pg/ml
- ELISA Type:
- Competitive ELISA, Coated with Antigen
- Synonyms:
- CRH, Corticotropin Releasing Hormone, Corticoliberin, Corticotropin-releasing factor, CRF, Corticotropin-releasing hormone
- Reactivity:
- Human
Frequently bought together:
Description
| 商品名: | Human CRH (Corticotropin Releasing Hormone) ELISA Kit |
| 製品コード: | HUFI00818 |
| サイズ: | 96T |
| エイリアス: | CRH, Corticotropin Releasing Hormone, Corticoliberin, Corticotropin-releasing factor, CRF, Corticotropin-releasing hormone |
| 検出方法: | Competitive ELISA, Coated with Antibody |
| 申し込み: | This immunoassay kit allows for the in vitro quantitative determination of Human CRH concentrations in serum plasma and other biological fluids. |
| 感度: | 1.4pg/ml |
| 範囲: | 2.188-140pg/ml |
| 保管所: | 4°C for 6 months |
| ノート: | For Research Use Only |
| 回復: | Matrices listed below were spiked with certain level of Human CRH and the recovery rates were calculated by comparing the measured value to the expected amount of Human CRH in samples. | ||||||||||||||||
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| 直線性: | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human CRH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. | ||||||||||||||||
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| CV(%): | Intra-Assay: CV<8% Inter-Assay: CV<10% |
| 成分 | 量 | 保管所 |
| ELISA Microplate(Dismountable) | 8×12 strips | 4°C for 6 months |
| Lyophilized Standard | 2 | 4°C/-20°C |
| Sample/Standard Dilution Buffer | 20ml | 4°C |
| Biotin-labeled Antibody(Concentrated) | 60ul | 4°C (Protect from light) |
| Antibody Dilution Buffer | 10ml | 4°C |
| HRP-Streptavidin Conjugate(SABC) | 120ul | 4°C (Protect from light) |
| SABC Dilution Buffer | 10ml | 4°C |
| TMB Substrate | 10ml | 4°C (Protect from light) |
| Stop Solution | 10ml | 4°C |
| Wash Buffer(25X) | 30ml | 4°C |
| Plate Sealer | 5 | - |
必要なその他の材料と設備:
- Microplate reader with 450 nm wavelength filter
- Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipettetips
- Incubator
- Deionized or distilled water
- Absorbent paper
- Buffer resevoir
| Uniprot | P06850 |
| UniProt Protein Function: | CRH: This hormone from hypothalamus regulates the release of corticotropin from pituitary gland. Belongs to the sauvagine/corticotropin-releasing factor/urotensin I family. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 8q13 Cellular Component: extracellular space; cytoplasm; extracellular region; perikaryon Molecular Function:corticotropin-releasing hormone receptor 1 binding; neuropeptide hormone activity; protein binding; hormone activity; corticotropin-releasing hormone receptor 2 binding; adrenocorticotropin-releasing hormone activity; receptor binding Biological Process: negative regulation of circadian sleep/wake cycle, REM sleep; diterpenoid metabolic process; synaptic transmission, dopaminergic; hypothalamus development; positive regulation of cortisol secretion; induction of apoptosis by hormones; response to pain; female pregnancy; signal transduction; positive regulation of adrenocorticotropic hormone secretion; negative regulation of luteinizing hormone secretion; synaptic transmission; learning and/or memory; positive regulation of cell proliferation; positive regulation of cAMP biosynthetic process; feeding behavior; negative regulation of blood pressure; parturition; inflammatory response; response to electrical stimulus; associative learning; response to corticosterone stimulus; response to drug; adrenal gland development; response to ether; response to ethanol; long-term memory; response to estrogen stimulus; ion homeostasis; regulation of serotonin secretion; negative regulation of epinephrine secretion; positive regulation of protein amino acid phosphorylation; glucocorticoid biosynthetic process; lung development Disease: Acth Deficiency, Isolated |
| NCBI Summary: | Corticotropin-releasing hormone is secreted by the paraventricular nucleus (PVN) of the hypothalamus in response to stress. Marked reduction in this protein has been observed in association with Alzheimer disease and autosomal recessive hypothalamic corticotropin deficiency has multiple and potentially fatal metabolic consequences including hypoglycemia and hepatitis. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery. A rapid increase in circulating levels of the hormone occurs at the onset of parturition, suggesting that, in addition to its metabolic functions, this protein may act as a trigger for parturition. [provided by RefSeq, Apr 2010] |
| UniProt Code: | P06850 |
| NCBI GenInfo Identifier: | 117445 |
| NCBI Gene ID: | 1392 |
| NCBI Accession: | P06850.1 |
| UniProt Secondary Accession: | P06850,B3KQS4, |
| UniProt Related Accession: | P06850 |
| Molecular Weight: | 196 |
| NCBI Full Name: | Corticoliberin |
| NCBI Synonym Full Names: | corticotropin releasing hormone |
| NCBI Official Symbol: | CRH |
| NCBI Official Synonym Symbols: | CRF; CRH1 |
| NCBI Protein Information: | corticoliberin; corticotropin-releasing factor |
| UniProt Protein Name: | Corticoliberin |
| UniProt Synonym Protein Names: | Corticotropin-releasing factor; CRF; Corticotropin-releasing hormone |
| Protein Family: | Corticoliberin |
| UniProt Gene Name: | CRH |
| UniProt Entry Name: | CRF_HUMAN |
*ノート: プロトコルは、各バッチ/ロットに固有です。正しい手順については、キットに含まれているプロトコルに従ってください。
使用前に、TMB基質を37°Cで少なくとも30分間平衡化します。サンプルと試薬を希釈するときは、完全に均一に混合する必要があります。各テストの標準曲線をプロットすることをお勧めします。
| ステップ | プロトコル |
| 1. | Set standard, test sample and control (zero) wells on the pre-coatedplate respectively, and then, record their positions. It isrecommended to measure each standard and sample in duplicate. Washplate 2 times before adding standard, sample and control (zero) wells! |
| 2. | Add Sample and Biotin-detection antibody: Add 50µL of Standard, Blank or Sample per well. The blankwell is added with Sample Dilution Buffer. Immediately add 50 µL of biotin-labelled antibody workingsolution to each well. Cover with the plate sealer provided. Gently tap the plate to ensure thoroughmixing. Incubate for 45 minutes at 37°C. (Solutions are added to the bottom of micro-ELISA platewell, avoid touching plate walls and foaming). |
| 3. | Wash: Aspirate each well and wash, repeating the process three timesWash by filling each well with Wash Buffer (approximately 350µL)using a squirt bottle, multi-channel pipette, manifold dispenser orautomated washer. Complete removal of liquid at each step is essentialto good performance. After the last wash, remove any remaining WashBuffer by aspirating or decanting. Invert the plate and pat it againstthick clean absorbent paper. |
| 4. | HRP-Streptavidin Conjugate(SABC): Add 100µL of SABC workingsolution to each well. Cover with a new Plate sealer. Incubate for30minutes at 37°C. |
| 5. | Wash: Repeat the aspiration/wash process for five times. |
| 6. | TMB Substrate: Add 90µL of TMB Substrate to each well. Coverwith a new Plate sealer. Incubate for about 10-20 minutes at 37°C.Protect from light. The reaction time can be shortened or extendedaccording to the actual color change, but not more than 30minutes.When apparent gradient appeared in standard wells, you can terminatethe reaction. |
| 7. | Stop: Add 50µL of Stop Solution to each well. Color turn toyellow immediately. The adding order of stop solution should be as thesame as the substrate solution. |
| 8. | OD Measurement: Determine the optical density (OD Value) of each wellat once, using a microplate reader set to 450 nm. You should open themicroplate reader ahead, preheat the instrument, and set the testing parameters. |
ELISAアッセイを実施する場合、可能な限り最良の結果を達成するためにサンプルを準備することが重要です。以下に、さまざまなサンプルタイプのサンプルを準備するための手順のリストを示します。
| サンプルタイプ | プロトコル |
| 血清 | If using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. |
| プラズマ | Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit. |
| 尿および脳脊髄液 | Collect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid. |
| 細胞培養上清 | Collect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately. |
| 細胞溶解物 | Solubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C. |
| 組織ホモジネート | The preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C. |
| 組織溶解物 | Rinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C. |
| 母乳 | Collect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles. |
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