Human ADAMTS5 (A Disintegrin And Metalloproteinase With Thrombospondin 5) ELISA Kit
The Human ADAMTS5 (A Disintegrin and Metalloproteinase with Thrombospondin 5) ELISA Kit is a powerful tool designed for the accurate measurement of ADAMTS5 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research purposes.ADAMTS5 is a key enzyme involved in extracellular matrix degradation and tissue remodeling processes. Dysregulation of ADAMTS5 has been linked to various diseases, including arthritis, cardiovascular diseases, and cancer. By accurately quantifying ADAMTS5 levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.Overall, the Human ADAMTS5 ELISA Kit is a valuable asset for researchers studying the role of ADAMTS5 in health and disease, offering precise and reliable measurement of this important enzyme in human samples.
Product Name:
Human ADAMTS5 (A Disintegrin And Metalloproteinase With Thrombospondin 5) ELISA Kit
SKU:
HUES03562
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
system_update_altDatasheetHuman A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5) ELISA KitThe Human ADAMTS5 ELISA Kit is a powerful tool for researchers loo
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