Human ACP5 (Tartrate Resistant Acid Phosphatase 5) ELISA Kit
The Human ACP5 (Tartrate-Resistant Acid Phosphatase 5) ELISA Kit is specifically designed for the accurate and efficient detection of ACP5 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and reproducible results for a variety of research purposes.ACP5, also known as tartrate-resistant acid phosphatase 5, is an important enzyme involved in bone metabolism and osteoclast function. It plays a crucial role in bone resorption and remodeling processes, making it a key biomarker for studying bone-related diseases such as osteoporosis and osteopetrosis.With its reliable performance and ease of use, the Human ACP5 ELISA Kit is a valuable tool for researchers studying bone biology, bone disorders, and potential therapeutic interventions targeting the regulation of ACP5 levels.
Product Name:
Human ACP5 (Tartrate Resistant Acid Phosphatase 5) ELISA Kit
SKU:
HUES02565
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
