[KO Validated] HMGB1 Rabbit Polyclonal Antibody (CAB2553)

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SKU:
CAB2553
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
HMG1, HMG3, HMG-1, SBP-1, HMGB1
Research Use:
Immunology
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Description

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[KO Validated] HMGB1 Antibody (CAB2553)

The [KO Validated] HMGB1 Antibody (CAB2553) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is specifically validated for use in applications such as Western blotting. By binding to the HMGB1 protein, researchers can effectively detect and analyze its presence in various cell types, making it a versatile option for studies in immunology and oncology.HMGB1, also known as high mobility group box 1 protein, is a key player in the immune response and can actively regulate inflammation, cell differentiation, and cell death.

This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] HMGB1 Antibody
SKU:CAB2553
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:ARYE REMK TYIP PKGE TKKK FKDP NAPK RPPS AFFL FCSE YRPK IKGE HPGL SIGD VAKK LGEM WNNT AADD KQPY EKKA AKLK EKYE KDIA AYRA KGKP
Tested Applications:WB IHC-P ELISA
Recommended Dilution:
WB1:2000 - 1:6000
IHC-P1:100 - 1:400
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:HMG1, HMG3, HMG-1, SBP-1, HMGB1
Positive Sample:HeLa, Mouse brain
Cellular Localization:Cell Membrane, Chromosome, Cytoplasm, Endoplasmic Reticulum-Golgi Intermediate Compartment, Endosome, Extracellular Side, Nucleus, Peripheral Membrane Protein, Secreted.
Calculated MW:25kDa
Observed MW:29kDa

This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein.

Purification MethodAffinity purification
Gene ID3146
RRIDAB_2863012
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from wild type (WT) and HMGB1 knockout (KO) HeLa cells using [KO Validated] HMGB1 Rabbit pAb (CAB2553) at 1:6000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60 s.
Product ImageWestern blot analysis of lysates from Mouse brain using [KO Validated] HMGB1 Rabbit pAb (CAB2553) at 1:6000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60 s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human breast cancer using [KO Validated] HMGB1 Rabbit pAb (CAB2553) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse heart using [KO Validated] HMGB1 Rabbit pAb (CAB2553) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Mouse liver tissue using [KO Validated] HMGB1 Rabbit pAb (CAB2553) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
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