HDAC3 Rabbit Polyclonal Antibody (CAB12542)

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SKU:
CAB12542
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse, Rat
Synonyms:
HD3, RPD3, KDAC3, RPD3-2, HDAC3
Research Use:
Cell Biology
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Description

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HDAC3 Antibody (CAB12542)

The HDAC3 Antibody (CAB12542) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot and immunohistochemistry applications. By binding specifically to the HDAC3 protein, this antibody enables precise detection and analysis in a variety of cell types, making it a valuable asset for studies in epigenetics, cancer research, and drug development.HDAC3, a member of the histone deacetylase family, plays a crucial role in the modification of chromatin structure and gene transcription. Dysregulation of HDAC3 activity has been implicated in a range of diseases, including cancer, neurological disorders, and metabolic syndromes.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:HDAC3 Antibody
SKU:CAB12542
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:FEYF APDF TLHP DVST RIEN QNSR QYLD QIRQ TIFE NLKM LNHA PSVQ IHDV PADL LTYD RTDE ADAE ERGP EENY SRPE APNE FYDG DHDN DKES DVEI
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:HD3, RPD3, KDAC3, RPD3-2, HDAC3
Positive Sample:Mouse brain
Cellular Localization:Cytoplasm, Nucleus, Cytosol.
Calculated MW:49kDa
Observed MW:49kDa

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.

Purification MethodAffinity purification
Gene ID8841
RRIDAB_2759382
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using HDAC3 Rabbit pAb (CAB12542) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Product ImageWestern blot analysis of lysates from mouse brain, using HDAC3 Rabbit pAb (CAB12542) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Product ImageImmunofluorescence analysis of HeLa cells using HDAC3 Rabbit pAb (CAB12542) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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