Description
system_update_altDatasheet
| Product Name: | GeniePlex Human TNFSF/TNFRSF 12-Plex (96 Tests) |
| Product Code: | HUAMPM051 |
| Size: | 96 Tests |
| Reactivity: | Human |
| Analytes (12): | TNF-alpha, TNFRI/TNFRSF1A/sCD120a, TNFRII/TNFRSF1B/sCD120b, RANKL/TNFSF11/sCD254, OPG/TNFRSF11B, TRAIL/TNFSF10/sCD253, TWEAK/TNFSF12, OX40/TNFRSF4/sCD134, LIGHT/TNFSF14/sCD258, APRIL/TNFSF13, 4-1BB/TNFRSF9/sCD137, and GITR/TNFRSF18/sCD357 |
| Sensitivity (LODs): | TNF-alpha: N/A pg/mL, TNFRI/TNFRSF1A/sCD120a: N/A pg/mL, TNFRII/TNFRSF1B/sCD120b: N/A pg/mL, RANKL/TNFSF11/sCD254: N/A pg/mL, OPG/TNFRSF11B: N/A pg/mL, TRAIL/TNFSF10/sCD253: N/A pg/mL, TWEAK/TNFSF12: N/A pg/mL, OX40/TNFRSF4/sCD134: N/A pg/mL, LIGHT/TNFSF14/sCD258: N/A pg/mL, APRIL/TNFSF13: N/A pg/mL, 4-1BB/TNFRSF9/sCD137: < 5 pg/mL, GITR/TNFRSF18/sCD357: < 20 pg/mL |
| Sample types: | Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate |
| Assay Type: | Multiplex |
| Detection Method: | Flow Cytometry |
| Assay Time: | 2 hours |
| 1x Ab-conjugated beads (premixed): | anti-human TNF-alpha, anti-human TNFRI/TNFRSF1A/sCD120a, anti-human TNFRII/TNFRSF1B/sCD120b, anti-human RANKL/TNFSF11/sCD254, anti-human OPG/TNFRSF11B, anti-human TRAIL/TNFSF10/sCD253, anti-human TWEAK/TNFSF12, anti-human OX40/TNFRSF4/sCD134, anti-human LIGHT/TNFSF14/sCD258, anti-human APRIL/TNFSF13, S4P4 - anti-human 4-1BB/TNFRSF9/sCD137, S5P8 - anti-human GITR/TNFRSF18/sCD357 |
| 2x Biotin-detection Ab (premixed): | Biotin - anti-human TNF-alpha, Biotin - anti-human TNFRI/TNFRSF1A/sCD120a, Biotin - anti-human TNFRII/TNFRSF1B/sCD120b, Biotin - anti-human RANKL/TNFSF11/sCD254, Biotin - anti-human OPG/TNFRSF11B, Biotin - anti-human TRAIL/TNFSF10/sCD253, Biotin - anti-human TWEAK/TNFSF12, Biotin - anti-human OX40/TNFRSF4/sCD134, Biotin - anti-human LIGHT/TNFSF14/sCD258, Biotin - anti-human APRIL/TNFSF13, Biotin - anti-human 4-1BB/TNFRSF9/sCD137, Biotin - anti-human GITR/TNFRSF18/sCD357 |
| Buffers: | 2x NR dAb Diluent. One vial containing 1.5 mL of biotin detection antibody diluent. |
| Lyophilized Standard Mix: | Lyophilized Standard Mix- Human TNFSF/TNFRSF 12-Plex. Vials A, B and C containing lyophilized recombinant human TNF-alpha, TNFRI/TNFRSF1A/sCD120a, TNFRII/TNFRSF1B/sCD120b, RANKL/TNFSF11/sCD254, OPG/TNFRSF11B, TRAIL/TNFSF10/sCD253, TWEAK/TNFSF12, OX40/TNFRSF4/sCD134, LIGHT/TNFSF14/sCD258, APRIL/TNFSF13, 4-1BB/TNFRSF9/sCD137, GITR/TNFRSF18/sCD357. |
| LLOQs: | TNF-alpha: N/A pg/mL, TNFRI/TNFRSF1A/sCD120a: N/A pg/mL, TNFRII/TNFRSF1B/sCD120b: N/A pg/mL, RANKL/TNFSF11/sCD254: N/A pg/mL, OPG/TNFRSF11B: N/A pg/mL, TRAIL/TNFSF10/sCD253: N/A pg/mL, TWEAK/TNFSF12: N/A pg/mL, OX40/TNFRSF4/sCD134: N/A pg/mL, LIGHT/TNFSF14/sCD258: N/A pg/mL, APRIL/TNFSF13: N/A pg/mL, 4-1BB/TNFRSF9/sCD137: < 10 pg/mL, GITR/TNFRSF18/sCD357: < 50 pg/mL. |
| ULOQs: | TNF-alpha: N/A pg/mL, TNFRI/TNFRSF1A/sCD120a: N/A pg/mL, TNFRII/TNFRSF1B/sCD120b: N/A pg/mL, RANKL/TNFSF11/sCD254: N/A pg/mL, OPG/TNFRSF11B: N/A pg/mL, TRAIL/TNFSF10/sCD253: N/A pg/mL, TWEAK/TNFSF12: N/A pg/mL, OX40/TNFRSF4/sCD134: N/A pg/mL, LIGHT/TNFSF14/sCD258: N/A pg/mL, APRIL/TNFSF13: N/A pg/mL, 4-1BB/TNFRSF9/sCD137: > 5,000 pg/mL, GITR/TNFRSF18/sCD357: > 20,000 pg/mL. |
| Standard dose recovery: | 70-130% |
| Intra-assay CV: | Intra-assay CV: <10% |
| Inter-assay CV: | Inter-assay CV: <20% |
| Cross-reactivity of analytes in the Panel: | Negligible |
| Sample volume: | 15 µL/test |
*Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
A filter plate washer is required for the following protocol. For more information on GeniePlex click here.
| Step | Protocol |
| 1. | Prepare the filter plate template. |
| 2. | Vortex working bead suspension for 15 seconds. |
| 3. | Add 45 µL of capture bead working suspension to each well. |
| 4. | Remove buffer in the wells by using the Filter Plate Washer connected to a vacuum source. |
| 5. | Remove buffer in the wells by using the Filter Plate Washer connected to a vacuum source. |
| 6. | Add 30 µL of CCS, SPB or TL Assay Buffer to each sample well. |
| 7. | Add 15 µL of samples to each sample well. Add 45 µL of standards to each standard well. Cover the plate with a plate seal. |
| 8. | Incubate on the shaker (set at 700 rpm) for 60 min at room temperature. Protect from light. |
| 9. | Remove the plate seal. Remove solutions in the wells by using the Filter Plate Washer. |
| 10. | Remove solutions in the wells by using the Filter Plate Washer. |
| 11. | Wash the wells three times with 100µL 1x Wash Buffer. |
| 12. | Gently tap the plate bottom onto paper towels to remove residual buffer. |
| 13. | Add 25µL of biotinylated antibody working solution to each well. Cover with plate seal. |
| 14. | Incubate on the shaker (700 rpm) for 30 min at room temperature. Protect from light. |
| 15. | Remove plate seal. Remove solutions. Wash three times with 100 µL 1x Wash Buffer. |
| 16. | Gently tap the plate bottom onto paper towels. |
| 17. | Add 25µL of streptavidin-PE working solution to each well. Cover with plate seal. |
| 18. | Incubate on the shaker (700 rpm) for 20 min at room temperature. Protect from light. |
| 19. | Remove plate seal. Remove solutions. Wash twice with 100 µL 1x Wash Buffer. |
| 20. | Add 150-300µL of 1x Reading Buffer to each well to re-suspend beads. Cover with plate seal. |
| 21. | Shake for 30 seconds at 700 rpm. Read on a flow cytometer. |
GeniePlex Technology
GeniePlex uses a unique mix of antibody-coated encoded microparticles providing an ultra-sensitive technology for the quantitation of analytes.
