The ENO2 Antibody (CAB3118) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, has high reactivity with human samples and is validated for use in various applications including Western blotting.Gamma enolase is an important enzyme involved in the glycolytic pathway, as well as in non-glycolytic functions such as DNA binding and regulation of gene expression. Its expression has been linked to various diseases, including cancer and neurodegenerative disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ENO2 Antibody
SKU:
CAB3118
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NSE, HEL-S-279, ENO2
Positive Sample:
U-87MG, SH-SY5Y, Mouse brain, Mouse liver, Rat brain, Rat uterus
Cellular Localization:
Cell Membrane, Cytoplasm.
Calculated MW:
47kDa
Observed MW:
47kDa
This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
Purification Method
Affinity purification
Gene ID
2026
RRID
AB_2764916
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using ENO2 Rabbit pAb (CAB3118) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
