The FBXW2 Antibody (CAB15996) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, derived from rabbit polyclonal sources, is highly specific for human samples and is validated for use in Western blotting applications. By binding to the FBXW2 protein, this antibody enables researchers to detect and analyze FBXW2 expression in a variety of cell types, making it ideal for studies in cell biology and protein degradation pathways.FBXW2 is a critical regulator of protein degradation, playing a key role in the targeted destruction of specific proteins within the cell.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
FBXW2 Antibody
SKU:
CAB15996
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Md6, FBW2, Fwd2, FBXW2
Positive Sample:
Mouse kidney, Rat kidney, Jurkat
Cellular Localization:
Cytosol.
Calculated MW:
52kDa
Observed MW:
51kDa/52kDa
F-box proteins are an expanding family of eukaryotic proteins characterized by an approximately 40 amino acid motif, the F box. Some F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins. In fact, F-box proteins are one of the four subunits of ubiquitin protein ligases, called SCFs. SCF ligases bring ubiquitin conjugating enzymes to substrates that are specifically recruited by the different F-box proteins. Mammalian F-box proteins are classified into three groups based on the presence of either WD-40 repeats, leucine-rich repeats, or the presence or absence of other protein-protein interacting domains. This gene encodes the second identified member of the F-box gene family and contains multiple WD-40 repeats.
Purification Method
Affinity purification
Gene ID
26190
RRID
AB_2763436
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using FBXW2 Rabbit pAb (CAB15996) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of lysates from Jurkat cells using FBXW2 Rabbit pAb (CAB15996) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
