The ERK5 Monoclonal Antibody (CAB3948) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated from rabbit immunizations, exhibits high specificity and sensitivity towards human ERK5 protein, making it an ideal choice for Western blot applications.By targeting the ERK5 protein, this antibody allows for precise detection and analysis of ERK5 expression in different cell types, providing valuable insights into its function and regulation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ERK5 Monoclonal Antibody
SKU:
CAB3948
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0871
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BMK1, ERK4, ERK5, PRKM7
Positive Sample:
Mouse lung, Mouse brain, Rat brain, HeLa, 293T
Cellular Localization:
Cytoplasm, Nucleus, Pml Body.
Calculated MW:
88kDa
Observed MW:
115kDa
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors. In response to extracelluar signals, this kinase translocates to cell nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors. Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported.
Purification Method
Affinity purification
Gene ID
5598
RRID
AB_2863161
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ERK5 Rabbit mAb (CAB3948) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180 s.
Western blot analysis of various lysates using ERK5 Rabbit mAb (CAB3948) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 3min.
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