The EpCAM Antibody (CAB1177) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for use in various research applications.EpCAM, also known as epithelial cell adhesion molecule, is commonly overexpressed in cancer cells, making it a promising target for cancer therapy and diagnostics. The EpCAM Polyclonal Antibody enables the detection and analysis of this protein in a wide range of cell types, providing valuable insights into its function and potential roles in cancer progression.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
EpCAM Antibody
SKU:
CAB1177
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Cell Junction, Lateral Cell Membrane, Single-Pass Type I Membrane Protein, Tight Junction.
Calculated MW:
35kDa
Observed MW:
35-45kDa
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.
Purification Method
Affinity purification
Gene ID
4072
RRID
AB_2758746
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using EpCAM Rabbit pAb (CAB1177) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of A431 cells using EpCAM Rabbit pAb (CAB1177) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
