Emerin/EMD Rabbit Polyclonal Antibody (CAB1132)

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SKU:
CAB1132
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse
Synonyms:
STA, EDMD, LEMD5, Emerin/EMD
Research Use:
Cell Biology
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Description

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Emerin/EMD Antibody (CAB1132)

The Emerin/EMD Antibody (CAB1132) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, produced using rabbit immunization, is highly specific to human emerin and is validated for use in various applications, including Western blot and immunofluorescence.Emerin is involved in various cellular processes, including gene regulation, DNA repair, and cell signaling, making it a key player in nuclear organization and stability.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:Emerin/EMD Antibody
SKU:CAB1132
Size:20μL, 100μL
Reactivity:Human, Mouse
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MDNY ADLS DTEL TTLL RRYN IPHG PVVG STRR LYEK KIFE YETQ RRRL SPPS SSAA SSYS FSDL NSTR GDAD MYDL PKKE DALL YQSK GYND DYYE ESYF TTRT YGEP ESAG PSRA VRQS VTSF PDAD AFHH QVHD DDLL SSSE EECK DRER PMYG RDSA YQSI THYR PVSA SRSS LDLS YYPT SSST SFMS SSSS SSSW LTRR AIRP ENRA PGAG LGQD
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IF/ICC1:20 - 1:100
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:STA, EDMD, LEMD5, Emerin/EMD
Positive Sample:HeLa, Jurkat, PC-3, U-251MG, Y79
Cellular Localization:Nucleoplasmic Side, Nucleus Inner Membrane, Nucleus Outer Membrane, Single-Pass Membrane Protein.
Calculated MW:29kDa
Observed MW:35kDa

Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene.

Purification MethodAffinity purification
Gene ID2010
RRIDAB_2758507
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using Emerin/EMD Rabbit pAb (CAB1132) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human uterus using Emerin/EMD Rabbit pAb (CAB1132) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageConfocal immunofluorescence analysis of U-2 OS cells using Emerin/EMD Rabbit pAb (CAB1132) at dilution of 1:100. Blue: DAPI for nuclear staining.
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