The DNMT3B Antibody (CAB2899) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human DNMT3B and has been validated for use in Western blot applications.DNMT3B plays a crucial role in establishing and maintaining DNA methylation patterns, which are essential for gene expression and cellular function. Dysregulation of DNMT3B has been implicated in various diseases, including cancer and neurological disorders. By targeting DNMT3B with this antibody, researchers can investigate its expression levels and localization in different cell types, providing insights into its function and potential therapeutic implications.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DNMT3B Antibody
SKU:
CAB2899
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ICF, ICF1, FSHD4, M.HsaIIIB, DNMT3B
Positive Sample:
U-251MG, HCT116, mouse testis, C6
Cellular Localization:
Nucleus.
Calculated MW:
96kDa
Observed MW:
110kDa
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined.
Purification Method
Affinity purification
Gene ID
1789
RRID
AB_2764719
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using DNMT3B Rabbit pAb (CAB2899) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
Immunofluorescence analysis of C6 cells using DNMT3B Rabbit pAb (CAB2899) at dilution of 100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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