The CRY2 Antibody (CAB6891) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high specificity for Cryptochrome 2 and is validated for use in Western blot applications. By binding to Cryptochrome 2, this antibody enables the detection and analysis of the protein in various cell types, making it ideal for investigations in chronobiology and DNA damage response pathways.Cryptochrome 2 is a key player in the circadian clock system, influencing the timing of physiological processes and behaviors in response to light cues.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CRY2 Antibody
SKU:
CAB6891
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HCRY2, PHLL2, CRY2
Positive Sample:
HepG2, A-549, Rat heart
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
67kDa
Observed MW:
67kDa
This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
1408
RRID
AB_2767450
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CRY2 Rabbit pAb (CAB6891) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
