CLIC1 Rabbit Polyclonal Antibody (CAB13684)

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SKU:
CAB13684
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction
Reactivity:
Human, Mouse, Rat
Synonyms:
G6, CL1C1, NCC27, CLCNL1, CLIC1
Research Use:
Signal Transduction
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Description

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CLIC1 Antibody (CAB13684)

The CLIC1 Antibody (CAB13684) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By targeting the CLIC1 protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for investigations in cell biology and neuroscience.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:CLIC1 Antibody
SKU:CAB13684
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:NVTT VDTK RRTE TVQK LCPG GQLP FLLY GTEV HTDT NKIE EFLE AVLC PPRY PKLA ALNP ESNT AGLD IFAK FSAY IKNS NPAL NDNL EKGL LKAL KVLD NYLT SPLP EEVD ETSA EDEG VSQR KFLD GNEL TLAD CNLL PKLH IVQV VCKK YRGF TIPE AFRG VHRY LSNA YARE EFAS TCPD DEEI ELAY EQVA KALK
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:G6, CL1C1, NCC27, CLCNL1, CLIC1
Positive Sample:Jurkat, HeLa, HepG2, Rat kidney
Cellular Localization:Cell Membrane, Cytoplasm, Nucleus, Nucleus Membrane, Single-Pass Membrane Protein.
Calculated MW:27kDa
Observed MW:27kDa

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 1 is a member of the p64 family; the protein localizes principally to the cell nucleus and exhibits both nuclear and plasma membrane chloride ion channel activity.

Purification MethodAffinity purification
Gene ID1192
RRIDAB_2760545
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using CLIC1 Rabbit pAb (CAB13684) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Product ImageImmunofluorescence analysis of C2C12 cells using CLIC1 Rabbit pAb (CAB13684) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of HepG2 cells using CLIC1 Rabbit pAb (CAB13684) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of K-562 cells using CLIC1 Rabbit pAb (CAB13684) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Product ImageImmunofluorescence analysis of THP-1 cells using CLIC1 Rabbit pAb (CAB13684) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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