CD63 Monoclonal Antibody is a premium monoclonal that offers outstanding performance and reliability for demanding research applications. Rigorously validated for ELISA, WB, IHC, IF, FC, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human, Rabbit samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 50ul format to meet your experimental needs. For optimal performance, store at Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
Product Name:
CD63 Monoclonal Antibody
SKU:
MACO0630
Size:
50μl
Isotype:
IgG1
Host Species:
Mouse
Reactivity:
Human, Rabbit
Immunogen:
Recombinant Human CD63 antigen protein (103-203AA)
Immunogen Species:
Homo sapiens (Human)
Uniprot No:
P08962
Form:
Liquid
Tested Applications:
ELISAWBIHCIFFC
Recommended Dilution:
Application
Recommended Dilution
WB
WB：1:1000-1：8000
IHC
1:50-1:200
IF
1:50-1:200
FC
1:50-1:200
Synonyms:
CD63, MLA1, TSPAN30, CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, LAMP-3, Lysosome integral membrane protein 1, Limp1, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, Tspan-30, CD antigen CD63
Western Blot Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate All lanes CD63 antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 30-120 KD KDa Observed band size: 30-120 KD KDa Exposure time:1min
Western Blot Positive WB detected in: Raji whole cell lysate All lanes CD63 antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 30-120 KD KDa Observed band size: 30-120 KD KDa Exposure time:1min
Western Blot Positive WB detected in: A375 whole cell lysate, Rabbit spleen tissue All lanes CD63 antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 30-120 KD KDa Observed band size: 30-120 KD KDa Exposure time:1min`
IHC image of MACO0630 diluted at 1:500 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of MACO0630 diluted at 1:500 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of MACO0630 diluted at 1:500 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of A549 cells with MACO0630 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Immunofluorescence staining of Hela cells with MACO0630 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Immunofluorescence staining of MCF-7 cells with MACO0630 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Overlay histogram showing A549 cells stained with MACO0630 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Overlay histogram showing Hela cells stained with MACO0630 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Overlay histogram showing HepG2 cells stained with MACO0630 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Overlay histogram showing K562 cells stained with MACO0630 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
1. Exosomes extracted from Hela cells 2. Exosomes extracted from Hela cells 3. Exosomes extracted from urine
