The CD276 Polyclonal Antibody (CAB24668) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced from rabbit serum, is highly specific to human samples and has been validated for use in Western blot applications. By targeting the CD276 protein, researchers can accurately detect and analyze its expression in various cell types, making it ideal for investigations in immunology and cancer research.CD276, also known as B7-H3, is involved in immune regulation and has been linked to the development and progression of various cancers. Its ability to modulate immune responses makes it a promising target for the development of new therapeutic strategies in cancer treatment.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
CD276 Polyclonal Antibody
SKU:
CAB24668
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
B7H3, B7-H3, B7RP-2, 4Ig-B7-H3, CD276
Positive Sample:
U-2 OS, LNCaP
Cellular Localization:
Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
33kDa
Observed MW:
100kDa
The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
80381
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using CD276 Rabbit pAb (CAB24668) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC):Jurkat Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using CD276 Rabbit pAb (CAB24668) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using CD276 Rabbit pAb (CAB24668) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
