The Caspase-3 Antibody (CAB11319) is a high-quality antibody developed for reliable detection and analysis of target proteins. Caspase-3 is a critical enzyme in the apoptosis pathway, playing a key role in cell death and survival processes. This antibody, generated in rabbits, is highly specific for caspase-3 and has been validated for use in Western blotting applications.This antibody binds specifically to caspase-3 protein, allowing for accurate detection and analysis in a variety of cell types. Its high reactivity with human samples makes it an ideal choice for researchers studying apoptosis in the context of diseases such as cancer, neurodegenerative disorders, and cardiovascular diseases.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Caspase-3 Antibody
SKU:
CAB11319
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CPP32, SCA-1, CPP32B, Caspase-3
Positive Sample:
Jurkat
Cellular Localization:
Cytoplasm.
Calculated MW:
32kDa
Observed MW:
17kDa/19kDa/32kDa
The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.
Purification Method
Affinity purification
Gene ID
836
RRID
AB_2758506
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide,pH7.3.
Western blot analysis of various lysates using Caspase-3 Rabbit pAb (CAB11319) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using Caspase-3 Rabbit pAb (CAB11319) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of NIH/3T3 cells using Caspase-3 Rabbit pAb (CAB11319) at dilution of 1:300 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using Caspase-3 Rabbit pAb (CAB11319) at dilution of 1:300 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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