The CARD11 Antibody (CAB7140) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high specificity for human samples and has been validated for use in Western blot assays. By binding to the CARD11 protein, this antibody facilitates the detection and analysis of CARD11 in various cell types, making it an invaluable tool for studies in immunology and cancer research.CARD11 is a crucial component of the adaptive immune response, playing a pivotal role in T cell receptor signaling and lymphocyte activation.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CARD11 Antibody
SKU:
CAB7140
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PPBL, BENTA, BIMP3, IMD11, CARMA1, IMD11A, CARD11
Positive Sample:
Raji, Jurkat, Mouse thymus
Cellular Localization:
Cytoplasm, Membrane Raft.
Calculated MW:
133kDa
Observed MW:
133kDa
The protein encoded by this gene belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10.
Purification Method
Affinity purification
Gene ID
84433
RRID
AB_2767695
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CARD11 Rabbit pAb (CAB7140) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of A549 cells using CARD11 Rabbit pAb (CAB7140). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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