The CPB1 Antibody (CAB10486) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to carboxypeptidase B, this antibody enables the detection and analysis of the enzyme in various cell types, making it ideal for studies in enzymology, protein degradation pathways, and biomedical research.Carboxypeptidase B is known for its role in peptide cleavage and protein digestion, making it a key player in processes such as food digestion and protein turnover within cells.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CPB1 Antibody
SKU:
CAB10486
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CPB, PASP, PCPB, CPB1
Positive Sample:
SW620, Mouse pancreas, Mouse liver, Mouse spleen, Rat pancreas
Cellular Localization:
Secreted.
Calculated MW:
47kDa
Observed MW:
47kDa
Three different procarboxypeptidases A and two different procarboxypeptidases B have been isolated. The B1 and B2 forms differ from each other mainly in isoelectric point. Carboxypeptidase B1 is a highly tissue-specific protein and is a useful serum marker for acute pancreatitis and dysfunction of pancreatic transplants. It is not elevated in pancreatic carcinoma.
Purification Method
Affinity purification
Gene ID
1360
RRID
AB_2758035
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CPB1 Rabbit pAb (CAB10486) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
